Resonance Raman microspectroscopy in biology

Rejection of fluorescence background in resonance and spontaneous Raman microspectroscopy.

Raman spectroscopy is often plagued by a strong fluorescent background, particularly for biological samples. If a sample is excited with a train of ultrafast pulses, a system that can temporally separate spectrally overlapping signals on a picosecond timescale can isolate promptly arriving Raman scattered light from late-arriving fluorescence light. Here we discuss the construction and operatio...

Triple-resonance coherent anti-stokes Raman scattering microspectroscopy.

Fluorescence-free microscopy: A new nonlinear optical microspectroscopy technique, femtosecond (fs) triple-resonance coherent anti-Stokes Raman scattering, in which the amplitude and phase of input fs laser pulses are optimally shaped to be in triple resonance with the molecular electronic and vibrational transitions, generates a coherent nonlinear signal beam at a new color with a highest poss...

Raman Microspectroscopy of LiDNA Fibres

The conformation of low-salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x-ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low-salt LiDNA fibre soaked in e...

Parallel Raman microspectroscopy using programmable multipoint illumination.

We present a novel parallel Raman microspectroscopy scheme for simultaneously collecting Raman spectra from multiple points. This scheme is realized by projectinga multiple-point laser illumination pattern using a spatial light modulator (SLM) and wide-field Raman imaging collection. We demonstrate the performance of this scheme using uniform samples, trapped polymer microparticles and fixed po...

Confocal Raman Microspectroscopy; Applications in Cartilage Tissue Engineering